Homepage of Prof. Dr. Andrea Sinz

Address:

Institute of Pharmacy
Martin Luther University Halle-Wittenberg
Wolfgang-Langenbeck-Strasse 4
D-06120 Halle (Saale), Germany

Phone: +49-345-55 25170
Fax: +49-345-55 27026
Email: andrea.sinz (AT) pharmazie.uni-halle.de

PLEASE HAVE A LOOK AT THE OFFICIAL HOMEPAGE (which is frequently updated) !!
http://agsinz.pharmazie.uni-halle.de/

Since January 15, 2007: Full Professor (W3) for Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Germany.

12/2005: Habilitation ("Bioanalytical Chemistry") at the Faculty of Chemistry & Mineralogy, University of Leipzig, Germany. Title of habilitation thesis: 'Structural and Functional Characterization of Proteins by Mass Spectrometry'

10/2001 to 12/2006: Head of Junior Research Group ‘Protein-Ligand Interaction with Ion Cyclotron Resonance Mass Spectrometry’, BBZ (Biotechnological-Biomedical Center), Faculty of Chemistry & Mineralogy, University of Leipzig, Germany.

03/2001 to 08/2001: Research Scientist (Mass Spectrometry),
Proteome Center Rostock, University of Rostock, Germany.

10/2000 to 02/2001: Scientific Assistant (Bioanalytical Chemistry),
Institute for Analytical Chemistry, University of Giessen, Germany.

10/98 to 09/2000: Post-Doctoral Fellow,
National Institutes of Health, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, Laboratory of Physical Biology, Bethesda, MD, USA.

04/98 to 09/98: Post-Doctoral Fellow,
National Institutes of Health, National Eye Institute, Laboratory of Ocular Therapeutics, Bethesda, MD, USA.

07/94 to 12/97: Doctoral Candidate Researcher,
Institute of Pharmaceutical Chemistry, University of Marburg, Germany.

Ph.D. Thesis title: 'Compounds from Cytotoxic Extracts of Dasymaschalon sootepense Craib, Anomianthus dulcis (Dun.) Sinclair, and Ellipeiopsis cherrevensis Fries'.

11/93 to 04/94: Practical Pharmaceutical Training,
Bayer Inc., Leverkusen, Germany. Department of Analytical Research for Drugs in Clinical Trials.

05/93 to 10/93: Practical Pharmaceutical Training, ‘Apotheke in der Uhlandstrasse’, Tuebingen, Germany.

1988-1993: Studies in Pharmacy (Staatsexamen)
University of Tuebingen, Germany.

2006: Innovation Prize in Medicinal / Pharmaceutical Chemistry (GDCh Fachgruppe Medizinische Chemie, DPhG Fachgruppe Pharmazeutische/Medizinische Chemie)

2004: Mattauch-Herzog Award (DGMS - German Society for Mass Spectrometry)

2001: Fellows Award for Research Excellence (National Institutes of Health)

The goals of our research are to elucidate protein-protein interactions as well as interactions between proteins and small bioactive ligands with the aim for biomedical and pharmaceutical application. Methods employed comprise chemical cross-linking, gel electrophoresis, multi-dimensional liquid chromatography, bioinformatics, and mass spectrometry, including the use of an LTQ-Orbitrap mass spectrometer (ThermoFisher Scientific) and nano-HPLC (high-performance liquid chromatography) / MALDI (matrix-assisted laser desorption / ionization) TOF (time-of-flight) / TOF mass spectrometry (Ultraflex III, Bruker Daltonik). We also develop gel-free approaches for global proteome studies and determine non-covalent interactions by electrospray ionization (ESI) mass spectrometry. Novel analytical methods are developed as they are applied to the present problems.

Original Papers

Sproß, J. and Sinz, A., Immobilized Monolithic Enzyme Reactors for Application in Proteomics and Pharmaceutics, Anal. Bioanal. Chem. in press.

Krauth, F., Ihling, C., Rüttinger, H.H. and Sinz, A., Heterobifunctional Isotope-labeled Amine-Reactive Photo-Cross-Linker for Structural Investigation of Proteins by MALDI-TOF/TOF and ESI-LTQ-Orbitrap Mass Spectrometry, Rapid Commun. Mass Spectrom. 23, 2811-2818 (2009)

Dimova, K., Kalkhof, S., Pottratz, I., Ihling, C., Rodriguez, F., Liepold, T., Brose, N., Sinz, A.* and Jahn, O.*, Structural Insights into the Calmodulin / Munc13 Interaction by Cross-Linking and Mass Spectrometry, Biochemistry 48, 5908-5921 (2009)
* Corresponding authors

Bauer, K., Kretzschmar, A.K., Cvijic, H., Blumert, C., Löffler, D., Brocke-Heidrich, K., Schiene-Fischer, C., Fischer, G., Sinz, A., Clevenger, C.V. and Horn, F., Cyclophilins contribute to Stat3 signaling and survival of multiple myeloma cells, Oncogene, online available (June 2009)

Müller, M.Q., de Koning, L. J., Schmidt, A., Ihling, C., Syha, Y., Rau, O., Mechtler, K., Schubert-Zsilavecz, M. and Sinz, A., An Innovative Method to Study Receptor-Drug Interactions by Mass Spectrometry, J. Med. Chem. 52, 2875-2879 (2009)

MacDougall, D., Wachten, S., Ciruela, A., Sinz, A. and Cooper, D.M.F., Separate Elements within a Single IQ-like Motif in Adenylyl Cyclase Type 8 Impart Ca2+/CaM Stimulation and Autoinhibition, J. Biol. Chem. 284, 15573-15588 (2009)

Manolaridis, I., Mumtsidu, E., Konarev, P., Makhov, A.M., Fullerton, S.F., Sinz, A., Kalkhof, S., McGeehan, J.E., Carey, P.D., Griffith, J.D., Svergun, D. Kneale, G.G. and Tucker, P.A., Structural and Biophysical Characterization of the Components of the Herpes Simplex Virus-1 Replication Origin, J. Biol. Chem. 284, 16343-16353 (2009)

Kölbel. K., Ihling, C., Bellmann-Sickert, K., Neundorf, I., Beck-Sickinger, A.G., Sinz, A., Kühn, U. and Wahle, E., T ype I arginine methyl transferases PRMT1 and 3 act distributively, J. Biol. Chem. 284, 8274-8282 (2009)

Müller, M.Q., Roth, C., Sträter, N. and Sinz, A., Expression and Purification of the Ligand-Binding Domain of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha), Prot. Exp. Purif. 62, 185-189 (2008),
DOI information: 10.1016/j.pep.2008.07.016

Kalkhof, S. and Sinz, A., Chances and Pitfalls of Chemical Cross-Linking with Amine-Reactive N-Hydroxy Succinimide Esters, Anal. Bioanal. Chem. 392, 305-312 (2008)

Kalkhof, S., Haehn, S., Ihling, C., Smyth, N. and Sinz, A., Determination of Disulfide Bond Patterns in Laminin ß1 Chain N-Terminal Domains by Nano-High Performance Liquid Chromatography/Matrix-Assisted Laser Desorption/Ionization Time-of-Flight/Time-of-Flight Mass Spectrometry, Rapid Commun. Mass Spectrom. 22, 1933–1940 (2008)

Schulz, D.M., Kalkhof, S., Schmidt, A., Ihling, C., Stingl, C., Mechtler, K., Zschörnig, O. and Sinz, A., Annexin A2 / p11 Interaction: New insights Into Annexin A2 Tetramer Structure by Chemical Cross-Linking, High-Resolution Mass Spectrometry, and Computational Modeling, Proteins 69, 254-269 (2007)

Pluder, F., Sinz, A. and Beck-Sickinger, A.G., Proliferative Effect of Calcitonin Gene-Related Peptide is Induced by at least Five Proteins as Identified by Proteome Profiling, Chem. Biol. Drug. Des. 69, 14-22 (2007)

Stingl, C., Ihling, C., Ammerer, G., Sinz, A. and Mechtler, K., Application of Different Fragmentation Techniques for the Analysis of Phosphopeptides using a Hybrid Linear Ion Trap-FTICR Mass Spectrometer, Biochim. Biophys. Acta- Proteins and Proteomics 1764, 1842–1852 (2006)

Ihling, C., Schmidt, A., Kalkhof, S., Stingl, C., Mechtler, K., Haack, M., Schulz, D.M., Cooper, D.M.F., Beck-Sickinger, A.G. and Sinz, A., Isotope-Labeled Cross-Linkers and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Structural Analysis of a Protein / Peptide Complex, J. Am. Soc. Mass Spectrom. 17, 1100-1113 (2006)

Schmitt, A., Meiners, I., Schmitt, J., Nöller, J., Ihling, C., Münch, G., Sinz, A. and Nieber, K., Two analytical methods to study the interaction of AGEs with cell surface proteins, J. Biochem. Biophys. Methods 65, 121-136 (2005)

Sinz, A., Kalkhof, S. and Ihling, C., Mapping Protein Interfaces by a Trifunctional Cross-Linker Combined with MALDI-TOF and ESI-FTICR Mass Spectrometry, J. Am. Soc. Mass Spectrom. 16, 1921-1931 (2005)

Schmidt, A., Kalkhof, S., Ihling, C., Cooper, D.M.F. and Sinz, A., Mapping Protein Interfaces by Chemical Cross-Linking and FTICR Mass Spectrometry: Application to a Calmodulin / Adenylyl Cyclase 8 Peptide Complex, Eur. J. Mass Spectrom. 11, 525-534 (2005)

Ihling, C. and Sinz, A., Proteome Analysis of Escherichia coli using High-Performance Liquid Chromatography and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Proteomics 5, 2029-2042 (2005)

Kalkhof, S., Ihling, C., Mechtler, K. and Sinz, A., Chemical Cross-Linking and High-Performance Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Protein Interaction Analysis: Application to a Calmodulin / Target Peptide Complex, Anal. Chem. 77, 495-503 (2005)

Berger, K., Wissmann, D., Ihling, C., Kalkhof, S., Beck-Sickinger, A., Sinz, A., Paschke, R., Führer, D., Quantitative proteome analysis in benign thyroid nodular disease using the fluorescent ruthenium II tris(bathophenanthroline disulfonate) stain, Mol. Cell. Endocrinol. 227, 21-30 (2004)

Sinz, A. and Wang, K., Mapping Spatial Proximities of Sulfhydryl Groups in Proteins Using a Fluorogenic Cross-Linker and Mass Spectrometry, Anal. Biochem. 331, 27-32 (2004)

Schulz, D.M., Ihling, C., Clore, G.M. and Sinz, A., Mapping the Topology and Determination of a Low Resolution Three-Dimensional Structure of the Calmodulin-Melittin Complex by Chemical Cross-Linking and High-Resolution FTICR Mass Spectrometry: Direct Demonstration of Multiple Binding Modes, Biochemistry 43, 4703-4715 (2004)

Schulz, D.M. and Sinz, A., Chemisches Cross-Linking und Massenspektrometrie zur strukturellen und funktionellen Charakterisierung von Proteinen, BIOSpektrum 10, 45-48 (2004)

Sinz, A., Chemical Cross-Linking and Mass Spectrometry for Mapping Three-Dimensional Structures of Proteins and Protein Complexes, J. Mass Spectrom. 38, 1225-1237 (2003)

Sinz, A., Jin, A.J. and Zschörnig, O., Evaluation of the Metal Binding Properties of a Histidine-Rich Fusogenic Peptide by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, J. Mass Spectrom. 38, 1150-1159 (2003)

Dihazi, G.H. and Sinz, A., Mapping Low-Resolution Three-Dimensional Protein Structures Using Chemical Cross-Linking and Fourier Transform Ion-Cyclotron Resonance Mass Spectrometry, Rapid Commun. Mass Spectrom. 17, 2005-2014 (2003)

Ihling, C., Berger, K., Höfliger, M.M., Führer, D., Beck-Sickinger, A.G. and Sinz, A., Nano-High-Performance Liquid Chromatography Combined with Nano-Electrospray Ionization Fourier Transform Ion-Cyclotron Resonance Mass Spectrometry for Proteome Analysis, Rapid Commun. Mass Spectrom. 17, 1240-1246 (2003)

Sinz, A., Bantscheff, M., Mikkat, S., Ringel, B., Drynda, S., Kekow, J., Thiesen, H.J. and Glocker, M.O., Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritis, Electrophoresis 23, 3445-3456 (2002)

Sinz, A. and Wang, K., Mapping protein interfaces with a fluorogenic cross-linker and mass spectrometry: Application to nebulin-calmodulin complexes, Biochemistry 40, 7903-7913 (2001)

Kawamura, M., Eisenhofer, G., Kopin, I.J., Kador, P.F., Lee, Y.S., Tsai, J.-Y., Fujisawa, S., Lizak, M.J., Sinz, A. and Sato, S., Aldose reductase: A key enzyme in norepinephrine metabolism in rats, Biochem. Pharmacol. 58, 517-524 (1999)

Sinz, A., Matusch, R., van Elsaecker, F., Santisuk, T., Chaichana, S. and Reutrakul, V., Phenolic Compounds from Anomianthus dulcis, Phytochemistry 50, 1069-1072 (1999)

Sinz, A., Matusch, R., Kaempchen, T., Fiedler, W., Schmidt, J., Santisuk, T., Chaichana, S., Reutrakul, V. and Wangcharoentrakul, S., Novel Acetogenins from the Leaves of Dasymaschalon sootepense, Helv. Chim. Acta 81, 1608-1615 (1998)

Sinz, A., Matusch, R., Santisuk, T., Chaichana, S. and Reutrakul, V., Flavonol Glycosides from Dasymaschalon sootepense, Phytochemistry 47, 1393-1396 (1998)
 

Review Articles

Mouradov, D., King, G., Ross, I.L., Forwood, J.K., Hume, D.A., Sinz, A., Martin, J.L., Kobe, B, Huber, T., Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling, Methods Mol Biol. 426, 459-474 (2008)

Sinz, A., Investigation of Protein-Ligand Interactions by Mass Spectrometry, ChemMedChem 16, 425-431 (2007)

Sinz, A., Isotope-Labeled Photoaffinity Reagents and Mass Spectrometry to Identify Protein-Ligand Interactions (Highlight Artikel), Angew. Chem. Int. Ed. 46, 660–662 (2007); Angew. Chem. 119, 670–673 (2007)

Sinz, A., Chemical Cross-Linking and Mass Spectrometry to Map Three-Dimensional Protein Structures and Protein-Protein Interactions, Mass Spectrom. Rev. 25, 663-682 (2006)

Ihling, C. and Sinz, A., Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Protein Structure Analysis, J. Biomacromol. Mass Spectrom. 1, 37-53 (2005)

Sinz, A., Chemical Cross-Linking and FTICR Mass Spectrometry for Protein Structure Characterization, Anal. Bioanal. Chem. 381, 44-47 (2005)

Sinz, A., Chemical Cross-Linking and Mass Spectrometry for Mapping Three-Dimensional Structures of Proteins and Protein Complexes, J. Mass Spectrom. 38, 1225-1237 (2003)
 

Book Chapters

Ihling, C. and Sinz, A., Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Protein Structure Analysis, Principles of Biomacromolecular Mass Spectrometry, König, S (ed.), Nova Science Publishers, 2008 (ISBN 1-60021-063-5)

Sinz, A., Chemical Cross-Linking for Protein Structure Characterization, Encyclopedia of Mass Spectrometry, vol. 6, 822-830 (2007)

Sinz, A., Chemical Cross-Linking and Mass Spectrometry for Investigation of Protein-Protein Interactions, Mass Spectrometry of Protein Interactions, Downard, K.M. (ed.), Wiley-Interscience, 2007 (ISBN 978-0-471-79373-1)
 

Review Articles (without Peer-Review)

Sproß, J. and Sinz, A., HPLC mit monolithischen Säulen, Deutsche Apotheker Zeitung 30, 46-48 (2009)

Krauth, F. and Sinz, A.,  Doping – Arzneimittelmissbrauch der besonderen Art, Pharm. Zeitung 153, 3150-3153 (2008)

Sinz, A. and Deininger, S.O., Massenspektrometrie zur Detektion von Biomarkern, Pharm. Unserer Zeit 37, 280-281 (2008)

Sinz, A., Die Bedeutung der Mutterkorn-Alkaloide als Arzneistoffe, Pharm. Unserer Zeit 37, 306-309 (2008)

Sinz, A., Massenspektrometrie in der Proteomik, Deutsche Apotheker Zeitung 29, 41-45 (2006)

Ihling, C. and Sinz, A., FTICR-Massenspektrometrie in der Proteinanalytik, Bioforum 27, 56-57 (2004),
Spectrométrie de masse FTICR dans l’analyse des protéines, Bioforum France 3, 30-31 (2004)

Schulz, D.M. and Sinz, A., Chemisches Cross-Linking und Massenspektrometrie zur strukturellen und funktionellen Charakterisierung von Proteinen, BIOSpektrum 10, 45-48 (2004)
 

Müller, M., Ihling, C., de Koning, L.J., Aardema, R., Dekker, H.D., Syha, Y., Schubert-Zsilavecz, M., de Koster, C.G., and Sinz, A., Interactions of the Peroxisome Proliferator-Activated Receptor alpha (PPAR?) with Ligands Analyzed by Chemical Cross-Linking and Nano-HPLC/MALDI-TOF/TOF-MS and HPLC/ESI-FTICR-MS, 56th ASMS Conference on Mass Spectrometry and Allied Topics (2008) Denver, CO, USA

Schulz, D.M., Kalkhof, S., Schmidt, A., Ihling, C., Stingl, C., Mechtler, K., Zschörnig, O. and Sinz, A., Annexin A2 / p11 Interaction: New insights Into Annexin A2 Tetramer Structure by Chemical Cross-Linking, High-Resolution Mass Spectrometry, and Computational Modeling, 56th ASMS Conference on Mass Spectrometry and Allied Topics (2008) Denver, CO, USA

Sinz, A., Schulz, D.M., Kalkhof, S., Schmidt, A., Ihling, C., Stingl, C. and Mechtler, K., Protein Interaction Studies by Chemical Cross-Linking Using Isotope-Labeled and Trifunctional Cross-Linkers Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, 54th ASMS Conference on Mass Spectrometry and Allied Topics (2006)

Kalkhof, S., Haehn, S., Ihling, C., Smyth, N., Meiler, J. and Sinz, A., Probing Laminin Self-Interaction by Chemical Cross-Linking and ESI-FTICR Mass Spectrometry, Proceedings of the 54th ASMS Conference on Mass Spectrometry and Allied Topics (2006)

Sinz, A., Kalkhof, S. and Ihling, C., Mapping Protein Interfaces Using a Trifunctional Cross-Linker Combined with MALDI-TOF and ESI-FTICR Mass Spectrometry, Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics (2005)

Schmidt, A., Kalkhof, S., Ihling, C., Schulz, D.M., Beck-Sickinger, A.G., Cooper, D.M.F. and Sinz, A., Studying Calmodulin / Adenylyl Cyclase 8 Interaction Using Isotope-Labeled Cross-Linkers and FTICR Mass Spectrometry, Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics (2005)

Kalkhof, S., Haehn, S., Ihling, C., Smyth, N. and Sinz, A., Probing Laminin Self-Interaction Using Isotope-Labeled Cross-Linkers and ESI-FTICR Mass Spectrometry, Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics (2005)

Sinz, A., Schulz, D.M., Ihling, C., Kalkhof, S. and Clore, G.M., Chemical Cross-Linking and FTICR Mass Spectrometry for Structural Modeling of Calmodulin / Peptide Complexes, Proceedings of the 52ndASMS Conference on Mass Spectrometry and Allied Topics (2004)

Schulz, D.M., Ihling, C., Clore, G.M. and Sinz, A., Elucidation of Low-Resolution Three-Dimensional Structures of the Calmodulin / Melittin Complex  by Chemical Cross-Linking and FTICR Mass Spectrometry, Proceedings of the 52ndASMS Conference on Mass Spectrometry and Allied Topics (2004)

Sinz, A., Dihazi, G.H., Schulz, D.M. and Ihling, C., Three-Dimensional Structure Determination of Proteins and Protein Complexes by Chemical Cross-Linking and Nano-HPLC / Nano-ESI FTICR Mass Spectrometry, Proceedings of the 51st ASMS Conference on Mass Spectrometry and Allied Topics (2003)

Sinz, A., Götze, L., Ringel, B., Spengler, B., Thiesen, H.J. and Glocker, M.O., Identification of Fibrinogen Degradation Products in Synovial Fluid of Patients with Different Forms of Arthritis by Mass Spectrometric Proteome Analysis. Proceedings of the 49th ASMS Conference on Mass Spectrometry and Allied Topics (2001)

Sinz, A. and Wang, K., Profiling Protein Interfaces by Chemical Cross-Linking: The Application of a Fluorogenic Cross-Linker and Mass Spectrometry. Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics (2000)

Sinz, A. and Wang, K., Non-covalent complexes of nebulin peptides with Ca²⁺ and other divalent cations examined by electrospray ionization mass spectrometry. Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics (1999)